At University of Copenhagen, Faculty of Life Sciences, we have recently started a PhD project on chemical profiling of polycyclic aromatic hydrocarbons (PAHs) and PAH metabolites in aqueous leachates from oil and tar contaminated soils.
The project will include three work packages:
1)Development of an analytical method for chemical profiling of PAHs and PAH metabolites in aqueous leachates. The method includes sample preparation using solid-phase extraction (SPE) and sample separation and detection by ultra-performance liquid chromatography (UPLC) interfaced to both a hybrid quadrupole-orthogonal acceleration Time-of-Flight (Q-TOF) mass spectrometer and to a fluorescence detector. The method will be validated analyzing leachates samples collected from organic contaminated soils located at Copenhagen Recycling Centre.
2)Small scale transportation experiments in the laboratory using both organic contaminated soils from Copenhagen Recycling Centre and soils spiked with a standard mixture of 30 compounds ranging from PAHs to hydroxylated and conjugated PAH metabolites.
3)Long-term lysimeter trials located at Copenhagen Recycling Centre including three soils with different contamination profiles – i.e. a tar, an oil and a diffuse traffic contaminated soil. The lysimeter work package includes chemical profiling of the soils and monitoring of PAHs and PAH metabolites in the aqueous leachates from the lysimeter.
For further information and/or comments, please contact PhD student, Esther Boll (eboll@life.ku.dk) or Ass. Prof. Jan H. Christensen (jch@life.ku.dk)
The project will include three work packages:
1)Development of an analytical method for chemical profiling of PAHs and PAH metabolites in aqueous leachates. The method includes sample preparation using solid-phase extraction (SPE) and sample separation and detection by ultra-performance liquid chromatography (UPLC) interfaced to both a hybrid quadrupole-orthogonal acceleration Time-of-Flight (Q-TOF) mass spectrometer and to a fluorescence detector. The method will be validated analyzing leachates samples collected from organic contaminated soils located at Copenhagen Recycling Centre.
2)Small scale transportation experiments in the laboratory using both organic contaminated soils from Copenhagen Recycling Centre and soils spiked with a standard mixture of 30 compounds ranging from PAHs to hydroxylated and conjugated PAH metabolites.
3)Long-term lysimeter trials located at Copenhagen Recycling Centre including three soils with different contamination profiles – i.e. a tar, an oil and a diffuse traffic contaminated soil. The lysimeter work package includes chemical profiling of the soils and monitoring of PAHs and PAH metabolites in the aqueous leachates from the lysimeter.
For further information and/or comments, please contact PhD student, Esther Boll (eboll@life.ku.dk) or Ass. Prof. Jan H. Christensen (jch@life.ku.dk)
